HPLC Preparative
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توضیحات
HPLC Preparative
دستگاه HPLC Preparative برای جداسازی و تخلیص purification نمونه ها
HPLC Preparative 200 mL/min
HPLC Semi-Preparative 50 mL/min
HPLC Analytical : 0.01 to 10 mL/min
1290 Infinity II Autoscale Preparative LC System یک راه حل مقیاس پذیر و مبتنی بر گردش کار است که مقیاس خودکار را از جستجوی متود آنالیتیکال تا تخلیص purification بهینه را تسهیل می کند.
افزایش مستقیم مقیاس scale-up از جستجوی تحلیلی تا خالص سازی purification در مقیاس گرم، امکان انتقال بدون درز در فرآیند توسعه دارو را فراهم می کند.
این افزایش مقیاس تضمین می کند که روش ها و شرایط خالص سازی purification بهینه شده در مقیاس تحلیلی می توانند به طور قابل اعتماد و کارآمد در مقادیر بیشتری از ترکیب مورد نظر اعمال شوند.
نرم‌افزار Automated Purification Software Agilent برای OpenLab ChemStation انتقال خودکار روش‌های تخلیص purification را از آنالیتیکال به خالص سازی purification در مقیاس آماده‌سازی امکان‌پذیر می‌سازد.
گرادیان های متمرکز برای هر ترکیب هدف target محاسبه می شوند و از بالاترین خلوص و بازیابی همه collected fractions اطمینان حاصل می کنند.
مزایای دستگاه HPLC Preparative :
- تزریق نمونه و fraction collection کاملاً اتوماتیک و ترکیبی
- دارای رنج فلو گسترده 1-200 mL/min
- دارای 10 جایگاه Column Capacity
- ظرفیت تزریق inject تا 864 نمونه یاcollect up fraction تا 432 فراکسیون یا هر ترکیب دیگر برای انعطاف پذیری کامل
- افزایش مقیاس از HPLCآنالیتیکال در یک سیستم واحد برای به حداکثر رساندن خلوص maximize purityو ظرفیت شما برای افزایش توان روزانه شما امکان‌پذیر می‌سازد.
- نرخ جریان تا 200 میلی‌لیتر در دقیقه از ستون‌های 50 میلی‌متری id پشتیبانی می‌کند و تعداد تزریق‌های مورد نیاز را برای ترکیب خالص‌تر در واحد زمان کاهش می‌دهد.
- بالاترین دانسیته Highest fraction density per unit bench space
- پشتیبانی از گردش‌های کاری workflows خودکار از طریق اطلاعات توالی‌های نمونه در حالی که تنها بر ترکیبات هدف خود تمرکز می‌کنید تا زمان تحلیل مجدد و فرمول‌بندی مجدد collected fractions را به حداقل برسانید.
- سیستم تخلیص اتوماتیک automated scale purification system مقیاس بسیار قابل اعتماد و قوی توانایی شما را برای مدیریت تقاضای بار کاری در حال افزایش بهبود می بخشد.
- سرهای پمپ با قابلیت تغییر آسان، محدوده جریان دینامیکی تا 200 میلی لیتر در دقیقه با حداکثر 600 بار را برای کاربردهای همه جانبه در طیف گسترده ای از جریان های کاری آماده سازی فراهم می کند.
- fractioncollectorقابل ارتقا بر اساس تقاضای شما.
- فناوری سنسور Fraction Collector یکپارچه و خودکار برای افزایش خلوص و collected fractions
- کنترل و سازماندهی دریچه ها valves: 5 اسلات چند منظوره سازماندهی بهبود یافته دریچه ها valves ، fraction delay coils و Agilent 1290 Infinity II MS Flow Modulator را برای افزایش قابل توجه بهره وری فراهم می کند.
- ادغام تمام حالت‌های collection modes استاندارد و سازگاری با چندین منبع trigger ، سازگاری با متود تخلیص purification methods موجود را فراهم می‌کند.
- کمترین حجم تاخیر Lowest delay volume برای به حداقل رساندن پخش پیک peak dispersion و carryover برای ریکاوری و خلوص نمونه عالی.
- زیرساخت نرم افزار از طریق نسخه Agilent OpenLab CDS ChemStation برای عملکرد راحت و قابل اعتماد
- نرم افزار Automated purification software دری را به روی اتوماسیون جریان کار باز می کند تا از شرایط HPLC تحلیلی به مقدماتی بر اساس الگوریتم های علمی ارتقا یابد و نیاز به توسعه روش های زمان بر برای بهبود چرخه های خالص سازی روزانه را از بین ببرد.
- ظرفیت fraction نمونه مقیاس پذیر Scalable بر اساس نیاز شما تا حداکثر 1296 fractions
1290 Infinity II Preparative LC Systems
بهترین دستگاه HPLC Preparative برای جداسازی و تخلیص purification نمونه ها تا نرخ جریان 1-200 mL/min
برای حل تمام چالش های خود با خلوص purification ، ریکاوری و سرعت بالا، انعطاف پذیری فوق العاده ای دریافت کنید.
دستگاه HPLC Preparative سیستم کروماتوگرافی مایع آماده سازی 1290 Infinity II تجسم نسل بعدی برای LC آماده سازی است که عملکرد فوق العاده بالایی در تخلیص purification ارائه می دهد.
1260 Infinity II Preparative LC Systems
دستگاه HPLC Preparative با خالص سازی کار آمد و fraction collector مقرون به صرفه به بالاترین خلوص و بازیابی دست یابید.
از انعطاف پذیری بالا در fraction size با حفظ ریکاوری با دقت بالا بهره مند شوید.
1220 /1260 / 1290 Infinity II دستگاه HPLC Semi-Preparative & HPLC Preparative HPLC Analytical & در مقیاس آنالیتیکال سیستم های خالص سازی LC کروماتوگرافی مایع
برای خالص‌سازی پروتئین‌ها یا نمونه‌های حساس، مدل bio-inert را انتخاب کنید.
سرمایه گذاری مطمئن در آینده
InfinityLab LC Purification Solutions بر اساس یک پلت فرم مشترک است.
از میان سیستم های از پیش پیکربندی شده انتخاب کنید، یا راه حلی متناسب با نیازهای تخلیص خاص خود را برای کشف، تحقیق یا تولید در مقیاس کوچک انتخاب کنید.
مسیرهای ارتقا یکپارچه به شما امکان می دهد ظرفیت را برای برآورده کردن تقاضاهای در حال تغییر افزایش دهید.
Load & Lock Columns
Product Description Product Number Usage
123 MultiPacker Station Air-driven hydraulic packing station for 1, 2, and 3 inch Load & Lock columns PCG93LLSTAND123 P
Load & Lock Column 27 × 500 mm Maximum working pressure 134 bar; maximum sorbent capacity 90 grams;
with stainless steel water jacket
PCG93LL500X25WJ P
Load & Lock Column 50 × 500 mm Maximum working pressure 134 bar; maximum sorbent capacity 590 grams;
with stainless steel water jacket
PCG93LL500X50WJ P
Load & Lock Column 75 × 500 mm Maximum working pressure 134 bar; maximum sorbent capacity 1325 grams;
with stainless steel water jacket
PCG93LL500X75WJ P
Prep Columns
InfinityLab Poroshell 120 SB-C18 21.2 × 150 mm, 4 μm, 120 Å 670150-902 P
InfinityLab Poroshell 120 HPH-C18 21.2 × 150 mm, 4 μm, 100 Å 670150-702 P
InfinityLab ZORBAX Eclipse Plus C18 21.2 × 150 mm, 5 μm, 95 Å 595150-902 P
InfnityLab ZORBAX SB-C18 21.2 × 150 mm, 5 μm, 80 Å 585150-902 P
InfinityLab Pursuit XRs C18 21.2 × 150 mm, 5 μm, 100 Å INF6000150X212 P
InfinityLab Pursuit XRs C18 30 × 150 mm, 5 μm, 100 Å INF6000150X300 P
PLRP-S 25.0 × 150 mm, 8 μm, 100 Å PL1212-3800 P
PLRP-S 50.0 × 150 mm, 8 μm, 100 Å PL1712-3800 P
For a complete list of dimensions and chemistries, refer to
Bulk Media
C18 Media Prep-C18, 10 μm, 100 Å, 1000 g 420910-902 P
Silica Media Prep-Silica, 10 μm, 100 Å, 1000 g 420910-901 P
Polymeric Media PLRP-S, 8 μm, 100 Å, 1000 g PL1412-6800 P
1260 Infinity II Variable Wavelength Detector ± 2.5 μAU/cm noise; 120 Hz (single wavelength detection);
2.5 Hz (dual wavelength detection; 190 to 600 nm)
G7114A A/P
1260 Infinity II Diode Array Detector WR ± 7 μAU/cm noise; 120 Hz; spectra; eight signals; 190 to 950 nm G7115A A/P
1260 Infinity II Multiple Wavelength Detector ± 7 μAU/cm noise; 120 Hz; eight signals; 190 to 950 nm G7165A A/P
Special Detection
1260 Infinity II Evaporative Light
Scattering Detector
For subambient operation; flow range 0.2 to 5 mL/min; evaporator
temperature 25 to 120 °C; nebulizer temperature 25 to 90 °C
G4260B A/P
LC/MSD Mass selective detector; up to m/z 2000 G6125B A/P
LC/MSD XT Mass selective detector; up to m/z 3000 high mass range;
30 ms polarity switching; Agilent Jet Stream as option
G6135B A/P
Support Modules
1290 Infinity II MS Flow Modulator Suitable for flow rates up to 200 mL/min; maximum pressure 69 bar; supports
split ratios between 100:1 and 100,000:1 with split frequencies up to 2 Hz
G7170B P
1260 Infinity II Delay Coil Organizer Up to nine delay coils can be installed; includes leak sensor and pressure
relief valve for increased laboratory safety
G9324A P
Fraction Collection
1290 Infinity II Preparative Open-Bed
Fraction Collector
Up to 200 mL/min flow; delay sensor technology; capacity 432 fractions;
maximum tube height 150 mm; all materials in flow path inert
G7159B P
1260 Infinity II Preparative Valve-Based
Fraction Collector
Up to 200 mL/min flow; delay sensor technology; capacity 11 fractions;
all materials in flow path inert
G7166A P
1260 Infinity II Preparative Fraction Collector Up to 100 mL/min flow; delay sensor technology; capacity 215 fractions;
maximum tube height 100 mm
G1364E P
1260 Infinity II Analytical-Scale Fraction Collector Up to 10 mL/min flow; delay sensor technology; capacity 215 fractions;
maximum tube height 48 mm
G1364F A
1260 Infinity II Analytical-Scale Bio-Inert
Fraction Collector
Up to 10 mL/min flow; delay sensor technology; capacity 215 fractions;
maximum tube height 48 mm
G5664B A
1290 Infinity II Preparative
Open-Bed Sampler/Collector
Maximum flow rate 200 mL/min; up to 20 mL injection loops; maximum capacity up
to 9 microtiter plates, 432 fraction tubes or 360 vials, or combination for injection and
fraction collection
G7158B P
1260 Infinity II Preparative Autosampler Maximum flow rate 200 mL/min; 0.1 to 3600 μL injection from vials; capacity
132 × 2 mL vials in 2 trays, or 36 × 6 mL vials in 2 trays
G7157A P
1290 Infinity II Multisampler 0 to 20 μL or 0 to 100 μL single-stroke injection at 1300 bar; up to 1500 μL with
optional kits; sample capacity up to 432 vials and up to 16 microtiter plates;
multiwash option for less than 9 ppm carryover; optional sample thermostat available
(Option #101); dual-needle option for highest throughput and flexibility
G7167B A
1290 Infinity II Vialsampler 0.1 to 20 μL (optionally 40 μL); up to 120 μL with multidraw mode at 1300 bar;
sample capacity up to 132 × 2 mL vials; optional integrated column compartment for
column heating up to 80 °C; optional integrated sample thermostat for sample
temperature control in the range of 4 to 40 °C (Option #101)
G7129B A
1260 Infinity II Multisampler 0 to 100 μL single-stroke injection at 800 bar; up to 1800 μL with optional kits; sample
capacity up to 432 vials and up to 16 microtiter plates; multiwash option for less than
9 ppm carryover; optional sample thermostat available (Option #101); dual-needle
option for highest throughput and flexibility
G7167A A
1260 Infinity II Vialsampler 0.1 to 100 μL injection from vials at up to 600 bar; up to 1800 μL with optional kits;
optional integrated column compartment for column heating up to 80 °C; optional
integrated sample thermostat for sample temperature control in the range of
4 to 40 °C (Option #101)
G7129A A
1260 Infinity II Bio-Inert
Multisample
0 to 100 μL single-stroke injection at 600 bar; up to 1000 μL with optional kits; sample
capacity up to 432 vials and up to 16 plates; multiwash option for less than 9 ppm
carryover; optional integrated sample thermostat for sample temperature control in
the range of 4-40 °C (Option #101)
G5668A A
Column/Valve Compartments and Organization
1290 Infinity II Preparative
Column Compartment
Holds up to four analytical columns, up to six preparative columns, and valves;
includes leak sensor for increase laboratory safety
G7163B P
1290 Infinity II Multicolumn
Thermostat
20 degrees below ambient to 110 °C; up to four 30 cm columns and up to eight
10 cm columns; up to eight individual InfinityLab Quick Connect heat exchangers for
standard-flow, high-flow, and ultralow dispersion; optional valve drive for InfinityLab
Quick Change valve heads
G7116B A
1260 Infinity II Column Organizer Maximum capacity of three columns; holds a manual injector; includes leak sensor
for increase laboratory safety
G9328A P
1260 Infinity II Multicolumn Thermostat 10 degrees below ambient to 85 °C; up to four 30 cm columns; up to four individual
InfinityLab Quick Connect heat exchangers; optional valve drive for InfinityLab Quick
Change valve heads (up to 800 bar and up to 4-column selection without replumbing)
G7116A A
1290 Infinity II Preparative Binary Pump Up to 200 mL/min; 600 bar; 4.6 to 50 mm column id; exchangeable pump heads;
integrated active seals wash and SSV
G7161B P
1260 Infinity II Preparative Binary Pump Up to 50 mL/min; 420 bar; 4.6 to 30 mm column id; integrated active seals wash G7161A P
1260 Infinity II Isocratic Pump 600 bar up to 5 mL/min; 200 bar up to 10 mL/min; integrated degassing unit
and SSV as option
G7110B A/M
1260 Infinity II Binary Pump 600 bar up to 5 mL/min; integrated degassing unit G7112B A
1260 Infinity II Quaternary Pump 600 bar up to 5 mL/min; 200 bar up to 10 mL/min; integrated degassing unit G7111B A/M
1260 Infinity II Bio-Inert Pump 600 bar up to 5 mL/min; 200 bar up to 10 mL/min; integrated degassing unit
quaternary mixing; bio-inert flow path
G5654A A
Valve Solutions
1290 Infinity Valve Drive For enhanced injection modes and advanced valve-switching applications
such as online SPE
G1170A P
1260 Infinity II Cluster Valve For clustering up to three fraction collectors G9322A P
InfinityLab Quick Change 2-Position/10-Port
Prep Valve
Maximum pressure 600 bar; maximum flow rate 200 mL/min G4730A P
InfinityLab Quick Change 8-Position/9-Port
Prep Valve
Maximum pressure 600 bar; maximum flow rate 200 mL/min G4731A P
InfinityLab Quick Change 6-Position/14-Port
Prep Valve
Maximum pressure 600 bar; maximum flow rate 200 mL/min G4734B P
InfinityLab Quick Change 2-Position/14-Port
Prep Valve
Maximum pressure 600 bar; maximum flow rate 200 mL/min G4738A P
Sample Injection (Manual)
1260 Infinity II Manual Preparative Injector 20 μL to 20 mL injection loops G1328D P
1260 Infinity II Manual Injector 5 μL to 5 mL injection loops G1328C A
1260 Infinity II Bio-Inert Manual Injector 5 μL to 5 mL injection loops G5628A A
Quick Reference Guide
Agilent InfinityLab LC Purification Solutions
Agilent offers tailor-made, high-efficiency LC purification solutions ranging from analytical to preparative scale. This comprehensive portfolio of state-of-the-art LC purification instruments offers you scalable LC purification solutions to meet your needs and budget. InfinityLab columns and supplies are designed to work seamlessly with Agilent InfinityLab instruments, and draw on 40+ years of technological leadership.
With Agilent OpenLab software and support from Agilent CrossLab instrument and enterprise service plans, Agilent provides a complete purification solution that will make a real difference to your lab’s operational efficiency.
1290 Infinity II Preparative LC Systems – Purify with the new benchmark in efficiency
Aspire to maximum efficiency in your purification workflows. Get maximum flexibility to solve all your purification challenges with highest purity, recovery, and speed.
1260 Infinity II Preparative LC Systems – Benefit from efficient purification – every day
Achieve highest purity and recovery with the most affordable fraction collector. Get highest flexibility in fraction size while maintaining high-precision recovery.
1220/1260/1290 Infinity II Analytical-Scale LC Purification Systems – Experience the power of analytical compound isolation
(Only preparative-scale systems are covered in this guide)
Isolate your compounds of interest through high-performance separation and low‑dispersion fraction collection. Choose the bio-inert option for purification of your proteins or sensitive samples 2
Pumps
| PM* | Part Number | ||
| Supplies for 1260 Infinity II Preparative Binary Pump (G7161A) | |||
| Standard preventive maintenance parts | |||
| Preventive maintenance kit, for the 1260 Infinity II preparative binary pump (G7161A), and 1290 Infinity II preparative binary pump (G7161B) equipped with 50 mL/min pump heads | PM kit | G7161-68730 | |
| Solvent bottle inlet filter, preparative, 20 μm, SST, 2/pk | 2 each | 5023-3115 | |
| Filter assembly, preparative, 12.7 mm id, 2 μm, SST | 1 each | 5022-2192 | |
| Piston Seal, for 1260 Infinity II preparative binary pump and 1290 Infinity II preparative binary pump G7161B with AtP pump head | 4 each | 5067-4299 | |
| Seal wash PE for 1260 Infinity II preparative binary pump G7161A and for AtP pump head, 1/pk | 4 each | 5067-6587 | |
| Peristaltic pump with PharMed tubing | 1 each | 5065-4445 | |
| Extended preventive maintenance parts | |||
| Piston collar, 5 mm, for G7161A 1260 Infinity II preparative binary pump and G7161B 1290 Infinity II preparative binary pump with AtP pump head | G7161-60005 | ||
| Check valve assembly, inlet/outlet, for G7161A/B preparative pump, with 50 mL/min pump head | 5067-6642 | ||
| Purge valve prep | G7161-60036 | ||
| Solvent mixer | G1312-87330 | ||
| Silicone tubing, 1 mm id, 3 mm od, 5 m | 5065-9978 | ||
| Other supplies | |||
| Bottle head assembly for 1260/1290 Infinity II Preparative LC system | G7161-60042 | ||
| InfinityLab capillary kit, for flow rate range 4 to 8 mL/min or column with 9.4 mm id | 5067-7015 | ||
| InfinityLab capillary kit, for flow rate range 15 to 40 mL/min or column with 21.2 mm id | 5067-7016 | ||
| InfinityLab capillary kit, for flow rate range 40 to 80 mL/min or column with 30 mm id | 5067-7017 | ||
| InfinityLab Supplies for 1290 Infinity II Preparative Binary Pump G7161B | |||
| Standard preventive maintenance parts (200 mL/min flow pump heads) | |||
| Preventive maintenance kit 1290 Infinity II Preparative Binary Pump | PM kit | G7161-68740 | |
| Piston seal, for 1290 Infinity II preparative binary pump G7161B with preparative pump head | 4 each | 5067-6589 | |
| Seal wash PE for 1290 Infinity II preparative binary pump G7161B, 1/pk | 4 each | 5067-6588 | |
| Filter assembly, preparative, 12.7 mm id, 2 μm, SST | 1 each | 5022-2192 | |
| Peristaltic pump with PharMed tubing | 1 each | 5065-4445 | |
| PM* | Part Number | ||
| Standard preventive maintenance parts (optional 50 mL pump heads) | |||
| Preventive maintenance kit, for the 1260 Infinity II preparative binary pump (G7161A), and 1290 Infinity II preparative binary pump (G7161B) equipped with 50 mL/min pump heads | PM kit | G7161-68730 | |
| Piston Seal, for 1260 Infinity II preparative binary pump and 1290 Infinity II preparative binary pump G7161B with AtP pump head | 4 each/option depending | 5067-4299 | |
| Seal wash PE for 1260 Infinity II preparative binary pump G7161A and for ATP pump head, 1/pk | 4 each/option depending | 5067-6587 | |
| Solvent bottle inlet filter, preparative, 20 μm, SST, 2/pk | 2 each | 5023-3115 | |
| Extended preventive maintenance parts | |||
| Check valve assembly double seat, inlet/outlet | G7161-60052 | ||
| Piston, for G7161B 1290 Infinity II preparative binary pump with preparative pump head | G7161-60028 | ||
| Purge valve prep | G7161-60036 | ||
| Solvent Mixer | G1312-87330 | ||
| Silicone tubing, 1 mm id, 3 mm od, 5 m | 5065-9978 | ||
| Piston collar, 5 mm, for G7161A 1260 Infinity II preparative binary pump and G7161B 1290 Infinity II preparative binary pump with AtP pump head | G7161-60005 | ||
| Check valve assembly, inlet/outlet, for G7161A/B preparative pump, with 50 mL/min pump head | 5067-6642 | ||
| Other supplies | |||
| Bottle head assembly, for 1260/1290 Infinity II Preparative LC system | G7161-60042 | ||
| InfinityLab capillary kit, for flow rate range 4 to 8 mL/min or column with 9.4 mm id | 5067-7015 | ||
| InfinityLab capillary kit, for flow rate range 15 to 40 mL/min or column with 21.2 mm id | 5067-7016 | ||
| InfinityLab capillary kit, for flow rate range 40to 80 mL/min or column with 30 mm id | 5067-7017 | ||
| InfinityLab capillary kit, for flow rate range 80 to 200 mL/min or column with 50 mm id | 5067-7018 | ||
Quick Reference Guide
Agilent InfinityLab Supplies for the 1260 Infinity II Preparative LC System
Agilent Technologies is committed to optimizing your laboratory’s productivity, so we have produced this list of the most commonly ordered supplies and parts for the 1260 Infinity II Preparative LC System.
| Preparative Binary Pump supplies (G7161A) | PM* | Part number | |
| Bottle head assembly | G7161-60042 | ||
| Solvent inlet filter, stainless steel, 20 μm, 2/pk | 2 each | 5023-3115 | |
| Capillary kit, LC/UV 4-8 mL/min, for 9.4 or 10 mm id columns | 5067-6858 | ||
| Capillary kit, LC/UV 15-40 mL/min, for 21.2 mm id columns | 5067-6859 | ||
| Peristaltic pump, with Pharmed tubing | 1 each | 5065-4445 | |
| Seal wash tubing, 5 m | 5065-9978 | ||
| Filter frit, 2 μm | 1 each | 5022-2192 | |
| Piston seal (pump head, 50 mL) | 4 each | 5067-4299 | |
| Wash seal (pump head, 50 mL) | 4 each | 5067-6587 | |
| Piston (pump head, 50 mL) | G7161-60005 | ||
| Check valve cartridge (pump head, 50 mL) | 5067-6642 | ||
| PM kit, for 1260 Infinity II preparative binary pump, 50 mL/min pump heads | Kit | G7161-68730 | |
| Preparative Autosampler supplies (G7157A) | PM* | Part number | |
| Needle | 1 each | G7157-87201 | |
| Needle seat | 1 each | G7157-87101 | |
| Rotor seal injection valve | 1 each | 5068-0268 | |
| Peristaltic pump, with Pharmed tubing | 1 each | 5065-4445 | |
| Gripper finger caps, 15/pk | 1 each | 5063-6506 | |
| Sample loop | G7157-60515 | ||
| Multi draw loop kit, 5 mL, for G7157A | G7157-68711 | ||
| Drawer, for 66 x 2 mL vials | G7129-60010 | ||
| Drawer, for 18 x 6 mL vials | G7129-60110 | ||
| Drawer kit, for 100 x 2 mL vials | G7129-68210 | ||
| PM kit, 1260 Infinity II preparative autosampler | Kit | G7157-68730 | |
| * PM (preventive maintenance) relevant part information: – x each: amount of part needed per PM – kit: preventive maintenance kit that includes all standard parts for PM – optional: part required for PM only if referring option is installed | |||
HPLC Preparative
HPLC Preparative 200 mL/min
HPLC Semi-Preparative 50 mL/min
HPLC Analytical : 0.01 to 10 mL/min
دستگاه HPLC Preparative برای جداسازی و تخلیص purification نمونه ها
دستگاه HPLC Preparative تخلیص پروتئین, دستگاه HPLC Preparative Agilent,دستگاه fplc,
نیما –
سپاس